Where massive capital is heading
Huge amounts of capital from global leaders are flowing into Longevity Biotech.
The reason they are betting astronomical sums is simple.
They have recognized that this business could become one of the fastest-growing markets in the future bio industry.
The Longevity Biotech trend attracting the world’s wealthiest investors
Amazon’s Jeff Bezos invested a massive $3 billion in Altos Labs, a company focused on restoring cellular resilience.
OpenAI’s Sam Altman boldly backed Retro Biosciences with $180 million, a company aiming to reverse aging biology.
Coinbase’s Brian Armstrong founded NewLimit, which works on restoring the function of aging cells and recently secured a successful $130 million investment round.
Sources: Financial Times, MIT Technology Review, Fortune
Senolytics and Fisetin
At the center of this trend is senolytics.
As we age, senescent cells gradually accumulate in the body.
Senolytics are a smart anti-aging strategy that leaves healthy normal cells untouched,
while precisely targeting and removing only the aging cells that make the skin look older.
Among ten flavonoids, Fisetin was found to be the most effective compound for removing senescent cells.
Senolytic
An anti-aging strategy that selectively removes senescent cells to help restore the function of tissues and organs.
Fisetin
A plant-based flavonoid found in green parts of plants and fruit peels.
It is known for strong antioxidant and anti-inflammatory effects and may help suppress certain aging-related processes.
Through its senolytic function of selectively removing senescent cells, it may contribute to slowing continuous aging.
In animal studies, it has been shown to reduce the incidence of age-related diseases and increase average lifespan.
Source: EBioMedicine 36 (2018) 18–28
Skincare with Fisetin
Fisenolyme
What if this powerful ingredient, Fisetin, could be used in cosmetics?
At CoSeedBioPharm, we developed Fisenolyme, a lime-derived ingredient packed with as much as 1,000 ppm of Fisetin.
Now, skin can be cared for with a powerful senolytic ingredient designed to target zombie cells.
Fisenolyme
Lime Extract
Citrus Aurantifolia (Lime) Fruit Extract
Senolytic Effect, Photoprotective, Anti-aging, Strengthening Skin Barrier
Can zombie cells really be cleared away?
Yes. In our experiment, skin aging markers visibly decreased by nearly half.
When the root cause of aging is reduced, it makes sense that the skin’s aging clock may slow down with continued use.
Senolytic Efficacy of Fisenolyme / Ex-vivo
The senescent cell-reducing effect of Fisenolyme was confirmed through p21 fluorescence staining.
After Fisenolyme treatment, the ratio of p21-positive cells decreased from 46% to 37.5%, showing an approximately 43% reduction.
While most nuclei in the control group showed strong red fluorescence, indicating a high level of senescence,
the density of red nuclei visibly decreased after Fisenolyme treatment.
Only the zombie cells, precisely targeted
Fisenolyme helps protect healthy cells, while accurately identifying senescent cells.
In the experiment, the signal that removes senescent cells became 26 times stronger than before.
In fact, this clearing signal was confirmed in 9 out of 10 senescent cells, or 90%.
Senolytic Efficacy of Fisenolyme / Ex-vivo
We evaluated whether Caspase-8, a key enzyme that directly induces apoptosis in senescent cells, was activated.
Unlike the control group, which showed no apoptotic signal, the Fisenolyme-treated group showed strong green fluorescence throughout the cytoplasm, proving activation of the apoptotic pathway.
Caspase-8 fluorescence intensity increased approximately 26-fold compared to the control group, quantitatively demonstrating excellent apoptosis-inducing efficacy.
To further verify whether Fisenolyme selectively eliminated senescent cells while protecting normal cells, two markers were merged and analyzed in detail.
In the Fisenolyme group, green apoptotic signals, Caspase-8, were clearly observed surrounding the remaining red senescent cell nuclei, p21+.
Notably, apoptotic signals were activated in as many as 90% of senescent cells, p21+, showing an approximately 98% increase compared to the control group and confirming precise targeting of senescent cells.
[Senescence-induced cells + lysis marker, nuclei/tissue(Control)]
[Fisenolyme-treated merged group]
Firm, juicy skin like fresh lime pulp
And it does not stop there.
After zombie cells are cleared away, the empty space is filled with collagen, the key to skin elasticity.
By helping control the factors that cause wrinkles, it delivers a plump, firm look that rises from within.
Anti-aging / Wrinkle-improving Efficacy of Fisenolyme
When treated with Fisenolyme at 10 μg/mL, the expression of COL1A1 and COL3A1,
the main collagen fibers in the dermis, increased by up to 85.2% and 98.0%, respectively.
In human dermal fibroblasts, it inhibited the expression of MMP1,
a wrinkle-inducing factor, by up to 50.5%, demonstrating excellent efficacy in suppressing skin aging.
Barrier-strengthening and Photoprotective Efficacy of Fisenolyme
Fisenolyme improves the expression of CerS3 and CerS4, genes essential for ceramide synthesis in the skin barrier,
suggesting potential support for long-term barrier function recovery.
Under UVA and UVB exposure, it reduces ROS generation and suppresses the expression of MMP,
MMP-1, and MMP-3, helping protect UV-damaged skin and prevent photoaging.
Sources: J. Soc. Cosmet. Sci. Korea Vol. 46, No. 4, December 2020, 391–401
J. Mol. Sci. 2023, 24, 17358
A 4 in1 all-round ingredient, Fisenolyme
Elasticity care, plus stronger barrier support and photoprotection.
It awakens the genes that help build a complete skin barrier and helps protect the skin from daily UV-induced damage and photoaging.
A true all-round ingredient that protects skin results from within.
Supercritical Extraction Applied
Fisenolyme uses supercritical extraction to maximize the efficacy of lime.
It gently and stably extracts heat-sensitive flavonoids, while using harmless CO₂
instead of organic solvents, making it an eco-friendly process.
Keeping active ingredients intact with an eco-friendly process
Moreover, with the supercritical extraction method, only the heat-sensitive Fisetin component is safely extracted without damage.
Because no chemical organic solvents are used, it is kind to both the body and the environment.
This makes it a storytelling-ready ingredient that can support a clean and complete product narrative.
4-week Stability Test
Fisenolyme contains 1,000 ppm of Fisetin as its main component and has been confirmed to remain stable after 4 weeks of stability testing.
No matter how good a high-content ingredient is, it means little if it easily breaks down inside a cosmetic formula.
Stability tested
Fisenolyme showed stable performance even under tough and persistent conditions.
It is a strong ingredient that survives well even in demanding formulas.
COSEED MAGAZINE
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Senolytic skincare Senolytic cosmetics
Senolytic ingredient Senolytic anti-aging
Senescent cell care Senescent cells in skin aging
Zombie cells skincare Zombie cell care
Targeting senescent cells Cellular anti-aging
Cell-level skincare Next-generation anti-aging Reverse aging skincare
Longevity beauty Longevity skincare Skin cellular senescence
Senescence-targeting skincare Lime-derived ingredient Lime extract skincare
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